Journal: Cell Reports

Article Title: A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs

doi: 10.1016/j.celrep.2018.12.074

Figure Lengend Snippet: Defects in DNA Damage Response in the syp-1 Phosphorylation Alleles (A) Sensitivity of L4-stage worms from the indicated strains to different doses of IR. Relative survival of offspring is shown. Data are represented as average percentage ± SD from at least four experiments with 15 worms each. ∗ p = 0.02, ∗∗ p = 0.0015, ∗∗∗ p = 0.0006, ∗∗∗∗ p < 0.0001; p values for paired t test. (B and C) Quantification of recombination marker RAD-51 foci in the indicated strains in normal conditions (B) or 20 h after 75 Gy (C). At least 15 gonads were analyzed in each condition and ten nuclei were scored in each zone (mitotic region, 1; transition zone, 2; early-mid-late pachytene regions, 3-4-5; and diplotene-diakinesis regions, 6) for at least three independent experiments. (D) Germ cell apoptosis was measured by differential interference contrast (DIC) microscopy in animals of the indicated strains at the indicated time points after IR treatment. Data are represented as average ± SD from at least ten worms for each time point of three independent experiments. ∗∗∗∗ p < 0.0001, p value for paired t test.

Article Snippet: Rabbit RAD-51 Antibody , Novus Biologicals , NB100-148.

Techniques: Marker, Microscopy

Journal: Cell Reports

Article Title: A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs

doi: 10.1016/j.celrep.2018.12.074

Figure Lengend Snippet:

Article Snippet: Rabbit RAD-51 Antibody , Novus Biologicals , NB100-148.

Techniques: Recombinant, Plasmid Preparation, Peptide Microarray, Clone Assay, Sequencing, Mutagenesis, Software, Fluorescence